x

inclusion body

uploads/inclusion body.jpg

inclusion body 【醫學】包涵體。

【醫學】包涵體。 “inclusion“ 中文翻譯：   n. 1.包含，含有。 2.參雜；雜質；內涵物。 3.【 ...“body“ 中文翻譯：   n. 1.身體，體軀，肉體；尸首；軀干，【林業】立木。 ...“body inclusion“ 中文翻譯：   包涵體“acute inclusion body encephalitis“ 中文翻譯：   急性包涵體腦炎“asteroid inclusion body“ 中文翻譯：   星狀包涵體“chlamydia inclusion body“ 中文翻譯：   衣原體包含體“dawson inclusion body encephalitis“ 中文翻譯：   道森氏包涵體腦炎“guarnieri inclusion body“ 中文翻譯：   瓜爾涅里包涵體,瓜尼瑞包涵體; 瓜尼瑞包涵體“inclusion body encephalitis“ 中文翻譯：   包涵體腦炎“inclusion body fibromatosis“ 中文翻譯：   包涵體纖維瘤病“inclusion body generation test“ 中文翻譯：   包涵體生成試驗“inclusion body hepatitis“ 中文翻譯：   包涵體肝炎“inclusion body hepatitis of chickens“ 中文翻譯：   雞包涵體肝炎“inclusion body myopathy“ 中文翻譯：   包涵體肌病“inclusion body myositis“ 中文翻譯：   包涵體性肌炎“inclusion body of hfrs virus“ 中文翻譯：   腎病綜合癥出血熱病毒包含體; 腎病綜合癥出血熱簿包含體“inclusion body of rhinitis“ 中文翻譯：   豬萎縮性鼻炎包涵體“inclusion-body disease virus“ 中文翻譯：   包含體病病毒; 包含體病簿,人皰疹簿5; 人皰疹病毒5“inclusion-body hepatitis virus“ 中文翻譯：   包含體肝炎病毒; 包含體肝炎簿“inclusion-body rhinitis“ 中文翻譯：   包涵體鼻炎“inclusion-body rhinitis virus“ 中文翻譯：   乳豬包含體鼻炎病毒; 乳豬包含體鼻炎簿“intracellular inclusion body“ 中文翻譯：   細胞內包涵體“irregular inclusion body“ 中文翻譯：   不規則包涵體“measles inclusion body encephalitis“ 中文翻譯：   麻疹包涵體腦炎“nuclear inclusion body“ 中文翻譯：   核包涵體

inclusive

The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga , 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm , this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青霉g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高于菌株dh5 pkkfpga 。

In this study , the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi , xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing , the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli , the transformed hosts were induced by iptg , bysds - page and elisa analysis of host protein . the expression of the objective gene was detected , and it could account for 16 . 28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg 同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，并轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。

Conclusion : it ' s possible to improve the expression efficiency by adjusting the g + c content of tir region and codon usage . replacement the cystein 78 and cystein 96 into serines , higher bioactivity gained compared with control hbfgf protein , and decreased amout of dimer and inclusion body , this suggested that free thiol group of cys78 and cys96 is the main reason of dimer and multimer formation 結論：通過調整tir區g c含量及增加大腸桿菌偏愛的密碼子，確實能提高hbfgf在大腸桿菌中的表達量，而將78和96位上的半瞇氨酸替換成絲氨酸后，得到的hbfgf的蛋白活性高于未改構蛋白，二聚體和包涵體減少。

After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant gst - eo protein by gst affinity columns . using the purified protein as coating antigen , an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum 使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。

The purified enzyme had a specific activity of 68 . 6 u / mg protein . overproduction of pga was often limited by translocation and / or periplasmic processing steps , subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm 經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。

The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e . coli culture contained protein 3a . the results of the study indicated that protein 3a existed in the form of inclusion body . the content of the expressed protein in the induced bacteria protein was 29 . 2 % and 22 . 1 % respectively Sds - page和westernbolt結果證實，大腸桿菌菌體不可溶性蛋白中富含3a蛋白，且此融合蛋白的分子量符合預期設計，說明3a蛋白在表達產物中以包涵體的形式存在，所表達的蛋白含量分別占菌體蛋白的29 . 2和22 . 1 。

The more favorable experiment conditions of preparing anatase nanometer tio2 powder are obtained from a lot of data . preparation technology of rutile nanometer tio2 powder is researched on the base of experiment of anatase nanometer tio2 powder . the influences of enclosure dose ' s quantity , preroasting temperatures phase - transition accelerant ' s quantity and calcining intensity and so on on the properties of inclusion body - zntio3 / ti ( oh ) 4 , granule size and properties of rutile nanometer tio2 powder are discussed 在銳鈦型納米tio _ 2粉體的制備基礎之上，進一步研究了金紅石型納米tio _ 2粉體的制備工藝，探討了包覆劑用量、預焙解溫度、晶型促進劑量及焙燒溫度和保溫時間等因素對znco _ 3 / ti ( oh ) _ 4沉淀包覆體性能、金紅石型納米tio _ 2粉體產品的粒度和性能的影響。

The recombinant b . thuringiensis subsp . israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant . it was found that b - pmpx2 strain remained stable toxicity , whatever during vegetative phase or sporulation phase , which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b . sphaericus 同時，將來源于蘇云金芽孢桿菌以色列亞種( b . thuringiensissubsp . israelensis , b . t . i )的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，并轉化到蘇云金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。

This subject uses the genetic engineering technology and the newest modern biotechnology to study the genetic engineering lower reaches stage , in order to research and establish the optimum , high density fermentation technology pattern and high efficient isolation and purification model of recombinant proteins from inclusion bodies 本課題利用基因工程技術，重點從基因工程下游階段著手研究，利用最新現代生物技術研究并建立經優化的高密度發酵工藝模式及高效分離純化包涵體重組蛋白模式。一

The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days . the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying . the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage . the chicken embryo dead in a week after it inoculated . the body was dropsy and hemorrhage . dead before it hatched out , the embyo body were dropsy , pale and slime . the liver was yellow and swolled , gallbladder ( vesica fellea ) was filled with bile . bursa and glandula thymus analosis . the kindey dropsy . bowel lamina were humble , dilatation . gas and yellow foam were filled the bowel . histopathological changes were that , in early stage , obvious changes of liver and kindey were dropsy , hemorrhage and necrosis . two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs . the obvious changes of bursa were dropsy , adverse folliiculated growth and little lymphocytes proliferating , 19 - 21 days chicken embryo , one or two big empty vacuoles were prensent in cells of liver and kindey . the number of the folliculi was growing , the vacuoles between cells were larger 膽囊充盈、其內充滿稀薄的膽汁；法氏囊、胸腺萎縮，腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物；腎臟腫大。病理組織學變化方面，早期肝臟、腎臟、腸主要以出血、水腫和壞死為主，且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體，包涵體呈嗜酸性，為大型圓形包涵體或不規則的顆粒狀；法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡，法氏囊濾泡數目增多細胞間有較大空隙。

Sectionii : to research and establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies . to raise separate efficiency and biological active efficiency is especially important in the purification model of recombinant proteins . in accordance with these questions , we raise inclusion bodies purity before the purification as far as possible , and select the suitable chromatogram technology 研究并建立一種包涵體中高效分離純化目的蛋白的優化模式重組蛋白分離純化工藝中分離效率的提高、生物活性效率的提高及工藝的穩定性是尤其重要的，針對這些問題，我們在層析純化前盡山西醫科大學生物化學與分子生物學2003屆碩士學位論文可能地提高包涵體純度，并選擇合適層析方法的同時合理的組合色譜分離單元。

After washing with reagent , adopt the newest purification technology source30rpc , sds - page and densitometric scan analysis , the result show that expression level is 90 % of total bacterial proteins . after renaturation , ifnr , hgfa , hgfb , hpk5 were purified by akta purifier chromatogram instrument , sepharose fast flow , ssphacrayl series gel , selecting optimize condition . finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies , purification product purity > 98 % 結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易于放大的包涵體重組蛋白分離純化體系。

Different hosts “ response suggested that tumv - sd1 could infect plants of 10 species in 3 families . tumv - sd1 formed pine - wheel inclusion bodies in plant cells . the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd , 38kd and 14kd respectively 寄主反應特性表明， tumv - sd1 6能侵染3科10種植物， tumv - sd1在寄主細胞內形成風輪狀內含體，外殼蛋白為3組分，分子量分別為45kd 、 38kd和14kd ；提純的病毒粒體為長線條狀。

Its content was about 9 . 8 % among total cell protein by gene genius bio imaging system . the fusion proteins were found largely in an insoluble inclusion bodies . the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105 經工ptg誘導，重組質粒在點co力‘ blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。

The virus could multifly on the mdck cells . and induce regular cytopathic effect ( cpe ) . observed by negative staining electron microscope , the virus could be seen with coroniform spike . in the ultrathin sections , the viruses were found to multiply and induce inclusion body forming in the cytoplasm 本研究從某動物園兩只死亡大熊貓的肝、脾等臟器分離病毒，經mdck細胞適應培養，獲得一株病毒(命名為dxmv ) ，在該細胞上可形成有規律的病變。

Although some pga precursors formed inclusion bodies in the cytoplasm of dh5a / pkkfpga , the amount only made up 5 - 10 % of all the afpga peptides , this explained why the expression level of afpga in dh5 / pkkfpga was a little lower than that in dh5 / psmlfpga 雖然青霉素g酰化酶在菌株dh5 pkkfpga中形成包涵體，但其量只占總酶的5 - 10 ，解釋了為什么菌株dh5 pkkfpga的表達量雖然比菌株dh5 pkkfpga低，但差距不大。

The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times , and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant 用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體，在37條件下，洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體，包涵體在8mol l尿素中的溶解性非常好。

After dissolving inclusion bodies , renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ) , the fusion protein trx - scfv of electrophoretic purity was obtained , they still possessed antigen - binding activity 經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。

After the expression form analysis , the insoluble recombinant proteins was purified by destraction and abstersion of inclusion bodies . to study the abstersion condition of the inclusion bodies , we adopted ultrasound crushing and freezing - melting methods 采用超聲加洗滌液破碎菌體；離心加凍融分離純化融合蛋白，研究不同的超聲次數和凍融對包涵體洗滌效果的影響。